1x first strand buffer (Thermo Fisher)
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1x first strand buffer
1x First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/first-strand+buffer/Tris+EDTA+buffer%2C+for+molecular+biology%2C+DNAse%2C+RNAse%2C+Protease+free+ready+to+use%2C+pH+8%2E0/pm42002808-95-17-21
Average 95 stars, based on 1 article reviews
1x First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/first-strand+buffer/Tris+EDTA+buffer%2C+for+molecular+biology%2C+DNAse%2C+RNAse%2C+Protease+free+ready+to+use%2C+pH+8%2E0/pm42002808-95-17-21
Average 95 stars, based on 1 article reviews
1x first strand buffer - by Bioz Stars,
2026-10
95/100 stars
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Incubation:Article Title: Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans Article Snippet: Next, 1 pmol of primer and 2 μg of small RNA fraction in 5 μL Article Title: Pseudouridine-modified tRNA fragments repress aberrant protein synthesis and predict leukaemic progression in myelodysplastic syndrome Article Snippet: The reaction was placed on ice for 2 min and RT mix was added to each sample (2.5 U μl −1 Article Title: Quiescence Modulates Stem Cell Maintenance and Regenerative Capacity in the Aging Brain. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GLAST-PE (ACSA-1) Miltenyi Biotec Cat# 130-098-804; RRID:AB_10829184 Anti-CD45-APC-Cy7 BD Cat# 557659; RRID:AB_396774 Anti-O4-APC (O4) Miltenyi Biotec Cat# 130-099-211; RRID: AB_2659794 Anti-CD133 ( Article Title: Method for nucleic acid amplification Article Snippet: On the other hand, the general composition of a reaction buffer used in reverse Article Title: Focal adhesion alterations in G0 ‐positive melanoma cells Article Snippet: Synthesis of a single cDNA strand was performed in a Article Title: The human tRNA-guanine transglycosylase displays promiscuous nucleobase preference but strict tRNA specificity Article Snippet: First strand cDNA synthesis was performed by addition of the RNA/primer mix to first-strand buffer (Thermofisher) containing 5 mM MgCl 2 , 0.1 M DTT, 4U SUPERase-In and 100U SuperScript III reverse transcriptase (ThermoFisher) with thermocycling: 25°C for 10 min, 50°C for 50 min, 85°C for 5 min. Article Title: Expression Analysis of Gnrh1 and Gnrhr1 in Spermatogenic Cells of Rat Article Snippet: Complementary DNA (cDNA) was obtained by reverse transcription using 5 μ g of total RNA, 0.5 μ g of oligo dT (18) , 0.5 mM dNTP mix, 5 mM DTT, 1x first-strand buffer (Invitrogen Life Technologies), 40 U Article Title: Infiltration of peripheral immune cells into the olfactory bulb in a mouse model of acute nasal inflammation. Article Snippet: Chronic nasal inflammation induces robust olfactory bulb (OB) atrophy in mice.. Here we examined initial events that occur in the OB after bilateral intranasal administration of lipopolysaccharide, focusing on the olfactory nerve fibers and meninges.. We analyzed the time course of OB and meninges inflammation using histological and biochemical approaches. Reverse Transcription:Article Title: Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans Article Snippet: Next, 1 pmol of primer and 2 μg of small RNA fraction in 5 μL Article Title: Pseudouridine-modified tRNA fragments repress aberrant protein synthesis and predict leukaemic progression in myelodysplastic syndrome Article Snippet: The reaction was placed on ice for 2 min and RT mix was added to each sample (2.5 U μl −1 Article Title: Quiescence Modulates Stem Cell Maintenance and Regenerative Capacity in the Aging Brain. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GLAST-PE (ACSA-1) Miltenyi Biotec Cat# 130-098-804; RRID:AB_10829184 Anti-CD45-APC-Cy7 BD Cat# 557659; RRID:AB_396774 Anti-O4-APC (O4) Miltenyi Biotec Cat# 130-099-211; RRID: AB_2659794 Anti-CD133 ( Article Title: Method for nucleic acid amplification Article Snippet: On the other hand, the general composition of a reaction buffer used in reverse Article Title: Focal adhesion alterations in G0 ‐positive melanoma cells Article Snippet: Synthesis of a single cDNA strand was performed in a Article Title: The human tRNA-guanine transglycosylase displays promiscuous nucleobase preference but strict tRNA specificity Article Snippet: First strand cDNA synthesis was performed by addition of the RNA/primer mix to first-strand buffer (Thermofisher) containing 5 mM MgCl 2 , 0.1 M DTT, 4U SUPERase-In and 100U SuperScript III reverse transcriptase (ThermoFisher) with thermocycling: 25°C for 10 min, 50°C for 50 min, 85°C for 5 min. Article Title: Expression Analysis of Gnrh1 and Gnrhr1 in Spermatogenic Cells of Rat Article Snippet: Complementary DNA (cDNA) was obtained by reverse transcription using 5 μ g of total RNA, 0.5 μ g of oligo dT (18) , 0.5 mM dNTP mix, 5 mM DTT, 1x first-strand buffer (Invitrogen Life Technologies), 40 U Article Title: Infiltration of peripheral immune cells into the olfactory bulb in a mouse model of acute nasal inflammation. Article Snippet: Chronic nasal inflammation induces robust olfactory bulb (OB) atrophy in mice.. Here we examined initial events that occur in the OB after bilateral intranasal administration of lipopolysaccharide, focusing on the olfactory nerve fibers and meninges.. We analyzed the time course of OB and meninges inflammation using histological and biochemical approaches. cDNA Synthesis:Article Title: Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans Article Snippet: Next, 1 pmol of primer and 2 μg of small RNA fraction in 5 μL Article Title: Pseudouridine-modified tRNA fragments repress aberrant protein synthesis and predict leukaemic progression in myelodysplastic syndrome Article Snippet: The reaction was placed on ice for 2 min and RT mix was added to each sample (2.5 U μl −1 Article Title: Quiescence Modulates Stem Cell Maintenance and Regenerative Capacity in the Aging Brain. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GLAST-PE (ACSA-1) Miltenyi Biotec Cat# 130-098-804; RRID:AB_10829184 Anti-CD45-APC-Cy7 BD Cat# 557659; RRID:AB_396774 Anti-O4-APC (O4) Miltenyi Biotec Cat# 130-099-211; RRID: AB_2659794 Anti-CD133 ( Article Title: Method for nucleic acid amplification Article Snippet: On the other hand, the general composition of a reaction buffer used in reverse Article Title: Focal adhesion alterations in G0 ‐positive melanoma cells Article Snippet: Synthesis of a single cDNA strand was performed in a Article Title: The human tRNA-guanine transglycosylase displays promiscuous nucleobase preference but strict tRNA specificity Article Snippet: First strand cDNA synthesis was performed by addition of the RNA/primer mix to first-strand buffer (Thermofisher) containing 5 mM MgCl 2 , 0.1 M DTT, 4U SUPERase-In and 100U SuperScript III reverse transcriptase (ThermoFisher) with thermocycling: 25°C for 10 min, 50°C for 50 min, 85°C for 5 min. Article Title: Expression Analysis of Gnrh1 and Gnrhr1 in Spermatogenic Cells of Rat Article Snippet: Complementary DNA (cDNA) was obtained by reverse transcription using 5 μ g of total RNA, 0.5 μ g of oligo dT (18) , 0.5 mM dNTP mix, 5 mM DTT, 1x first-strand buffer (Invitrogen Life Technologies), 40 U Article Title: Infiltration of peripheral immune cells into the olfactory bulb in a mouse model of acute nasal inflammation. Article Snippet: Chronic nasal inflammation induces robust olfactory bulb (OB) atrophy in mice.. Here we examined initial events that occur in the OB after bilateral intranasal administration of lipopolysaccharide, focusing on the olfactory nerve fibers and meninges.. We analyzed the time course of OB and meninges inflammation using histological and biochemical approaches. Real-time Polymerase Chain Reaction:Article Title: Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans Article Snippet: Next, 1 pmol of primer and 2 μg of small RNA fraction in 5 μL Article Title: Pseudouridine-modified tRNA fragments repress aberrant protein synthesis and predict leukaemic progression in myelodysplastic syndrome Article Snippet: The reaction was placed on ice for 2 min and RT mix was added to each sample (2.5 U μl −1 Article Title: Quiescence Modulates Stem Cell Maintenance and Regenerative Capacity in the Aging Brain. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GLAST-PE (ACSA-1) Miltenyi Biotec Cat# 130-098-804; RRID:AB_10829184 Anti-CD45-APC-Cy7 BD Cat# 557659; RRID:AB_396774 Anti-O4-APC (O4) Miltenyi Biotec Cat# 130-099-211; RRID: AB_2659794 Anti-CD133 ( Article Title: Method for nucleic acid amplification Article Snippet: On the other hand, the general composition of a reaction buffer used in reverse Article Title: Focal adhesion alterations in G0 ‐positive melanoma cells Article Snippet: Synthesis of a single cDNA strand was performed in a Article Title: The human tRNA-guanine transglycosylase displays promiscuous nucleobase preference but strict tRNA specificity Article Snippet: First strand cDNA synthesis was performed by addition of the RNA/primer mix to first-strand buffer (Thermofisher) containing 5 mM MgCl 2 , 0.1 M DTT, 4U SUPERase-In and 100U SuperScript III reverse transcriptase (ThermoFisher) with thermocycling: 25°C for 10 min, 50°C for 50 min, 85°C for 5 min. Article Title: Expression Analysis of Gnrh1 and Gnrhr1 in Spermatogenic Cells of Rat Article Snippet: Complementary DNA (cDNA) was obtained by reverse transcription using 5 μ g of total RNA, 0.5 μ g of oligo dT (18) , 0.5 mM dNTP mix, 5 mM DTT, 1x first-strand buffer (Invitrogen Life Technologies), 40 U Article Title: Infiltration of peripheral immune cells into the olfactory bulb in a mouse model of acute nasal inflammation. Article Snippet: Chronic nasal inflammation induces robust olfactory bulb (OB) atrophy in mice.. Here we examined initial events that occur in the OB after bilateral intranasal administration of lipopolysaccharide, focusing on the olfactory nerve fibers and meninges.. We analyzed the time course of OB and meninges inflammation using histological and biochemical approaches. Polymerase Chain Reaction:Article Title: Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans Article Snippet: Next, 1 pmol of primer and 2 μg of small RNA fraction in 5 μL Article Title: Pseudouridine-modified tRNA fragments repress aberrant protein synthesis and predict leukaemic progression in myelodysplastic syndrome Article Snippet: The reaction was placed on ice for 2 min and RT mix was added to each sample (2.5 U μl −1 Article Title: Quiescence Modulates Stem Cell Maintenance and Regenerative Capacity in the Aging Brain. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GLAST-PE (ACSA-1) Miltenyi Biotec Cat# 130-098-804; RRID:AB_10829184 Anti-CD45-APC-Cy7 BD Cat# 557659; RRID:AB_396774 Anti-O4-APC (O4) Miltenyi Biotec Cat# 130-099-211; RRID: AB_2659794 Anti-CD133 ( Article Title: Method for nucleic acid amplification Article Snippet: On the other hand, the general composition of a reaction buffer used in reverse Article Title: Focal adhesion alterations in G0 ‐positive melanoma cells Article Snippet: Synthesis of a single cDNA strand was performed in a Article Title: The human tRNA-guanine transglycosylase displays promiscuous nucleobase preference but strict tRNA specificity Article Snippet: First strand cDNA synthesis was performed by addition of the RNA/primer mix to first-strand buffer (Thermofisher) containing 5 mM MgCl 2 , 0.1 M DTT, 4U SUPERase-In and 100U SuperScript III reverse transcriptase (ThermoFisher) with thermocycling: 25°C for 10 min, 50°C for 50 min, 85°C for 5 min. Article Title: Expression Analysis of Gnrh1 and Gnrhr1 in Spermatogenic Cells of Rat Article Snippet: Complementary DNA (cDNA) was obtained by reverse transcription using 5 μ g of total RNA, 0.5 μ g of oligo dT (18) , 0.5 mM dNTP mix, 5 mM DTT, 1x first-strand buffer (Invitrogen Life Technologies), 40 U Article Title: Infiltration of peripheral immune cells into the olfactory bulb in a mouse model of acute nasal inflammation. Article Snippet: Chronic nasal inflammation induces robust olfactory bulb (OB) atrophy in mice.. Here we examined initial events that occur in the OB after bilateral intranasal administration of lipopolysaccharide, focusing on the olfactory nerve fibers and meninges.. We analyzed the time course of OB and meninges inflammation using histological and biochemical approaches. Control:Article Title: Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans Article Snippet: Next, 1 pmol of primer and 2 μg of small RNA fraction in 5 μL Article Title: Pseudouridine-modified tRNA fragments repress aberrant protein synthesis and predict leukaemic progression in myelodysplastic syndrome Article Snippet: The reaction was placed on ice for 2 min and RT mix was added to each sample (2.5 U μl −1 Article Title: Quiescence Modulates Stem Cell Maintenance and Regenerative Capacity in the Aging Brain. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GLAST-PE (ACSA-1) Miltenyi Biotec Cat# 130-098-804; RRID:AB_10829184 Anti-CD45-APC-Cy7 BD Cat# 557659; RRID:AB_396774 Anti-O4-APC (O4) Miltenyi Biotec Cat# 130-099-211; RRID: AB_2659794 Anti-CD133 ( Article Title: Method for nucleic acid amplification Article Snippet: On the other hand, the general composition of a reaction buffer used in reverse Article Title: Focal adhesion alterations in G0 ‐positive melanoma cells Article Snippet: Synthesis of a single cDNA strand was performed in a Article Title: The human tRNA-guanine transglycosylase displays promiscuous nucleobase preference but strict tRNA specificity Article Snippet: First strand cDNA synthesis was performed by addition of the RNA/primer mix to first-strand buffer (Thermofisher) containing 5 mM MgCl 2 , 0.1 M DTT, 4U SUPERase-In and 100U SuperScript III reverse transcriptase (ThermoFisher) with thermocycling: 25°C for 10 min, 50°C for 50 min, 85°C for 5 min. Article Title: Expression Analysis of Gnrh1 and Gnrhr1 in Spermatogenic Cells of Rat Article Snippet: Complementary DNA (cDNA) was obtained by reverse transcription using 5 μ g of total RNA, 0.5 μ g of oligo dT (18) , 0.5 mM dNTP mix, 5 mM DTT, 1x first-strand buffer (Invitrogen Life Technologies), 40 U Article Title: Infiltration of peripheral immune cells into the olfactory bulb in a mouse model of acute nasal inflammation. Article Snippet: Chronic nasal inflammation induces robust olfactory bulb (OB) atrophy in mice.. Here we examined initial events that occur in the OB after bilateral intranasal administration of lipopolysaccharide, focusing on the olfactory nerve fibers and meninges.. We analyzed the time course of OB and meninges inflammation using histological and biochemical approaches. Recombinant:Article Title: Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans Article Snippet: Next, 1 pmol of primer and 2 μg of small RNA fraction in 5 μL Article Title: Pseudouridine-modified tRNA fragments repress aberrant protein synthesis and predict leukaemic progression in myelodysplastic syndrome Article Snippet: The reaction was placed on ice for 2 min and RT mix was added to each sample (2.5 U μl −1 Article Title: Quiescence Modulates Stem Cell Maintenance and Regenerative Capacity in the Aging Brain. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GLAST-PE (ACSA-1) Miltenyi Biotec Cat# 130-098-804; RRID:AB_10829184 Anti-CD45-APC-Cy7 BD Cat# 557659; RRID:AB_396774 Anti-O4-APC (O4) Miltenyi Biotec Cat# 130-099-211; RRID: AB_2659794 Anti-CD133 ( Article Title: Method for nucleic acid amplification Article Snippet: On the other hand, the general composition of a reaction buffer used in reverse Article Title: Focal adhesion alterations in G0 ‐positive melanoma cells Article Snippet: Synthesis of a single cDNA strand was performed in a Article Title: The human tRNA-guanine transglycosylase displays promiscuous nucleobase preference but strict tRNA specificity Article Snippet: First strand cDNA synthesis was performed by addition of the RNA/primer mix to first-strand buffer (Thermofisher) containing 5 mM MgCl 2 , 0.1 M DTT, 4U SUPERase-In and 100U SuperScript III reverse transcriptase (ThermoFisher) with thermocycling: 25°C for 10 min, 50°C for 50 min, 85°C for 5 min. Article Title: Expression Analysis of Gnrh1 and Gnrhr1 in Spermatogenic Cells of Rat Article Snippet: Complementary DNA (cDNA) was obtained by reverse transcription using 5 μ g of total RNA, 0.5 μ g of oligo dT (18) , 0.5 mM dNTP mix, 5 mM DTT, 1x first-strand buffer (Invitrogen Life Technologies), 40 U Article Title: Infiltration of peripheral immune cells into the olfactory bulb in a mouse model of acute nasal inflammation. Article Snippet: Chronic nasal inflammation induces robust olfactory bulb (OB) atrophy in mice.. Here we examined initial events that occur in the OB after bilateral intranasal administration of lipopolysaccharide, focusing on the olfactory nerve fibers and meninges.. We analyzed the time course of OB and meninges inflammation using histological and biochemical approaches. |
